Category Archives: Journal Club

Journal Club: Connectomic reconstruction of the inner plexiform layer in the mouse Retina

In a landmark paper demonstrating the capabilities of modern connectomics approaches, Moritz Helmstaedter et al. present a reconstruction of nearly 1000 cells and 500,000 cell-cell contacts in the mouse Retina using data collected with serial block face microscopy. This is a high-throughput electron microscopy method championed by Moritz and two of the other co-authors, Kevin Briggman (now at NIH in Bethesda) and of course Winfried Denk (a Max Planck Director with joint appointment at Janelia Farm). Valuable in its own right, this paper represents Continue reading

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Journal Club: Connecting a Connectome to Behavior

0yks_X4B3uHScKQYMpmLDzl72eJkfbmt4t8yenImKBVaiQDB_Rd1H6kmuBWtceBJAn obvious goal of connectomics is to use it is a tool for better understanding complex network function. As new data are becoming available it is  not entirely clear what the best methods are for extracting useful information out of connectivity matrices. Part of the problem lies in the fact that while a wiring diagram is necessary for understanding nervous system function, it is hardly sufficient. The painful reality is that in complex systems like networks of neurons, small details of neuron and synapse function can dramatically alter system behavior. A recent Plos Computational Biology paper by Eduardo Izquierdo and Randall Beer at Indiana University makes what I think is a useful contribution to the problem by Continue reading